In the same year, Agnes' mother, Queen Constance of Hungary, bought the church of St. Peter in Poříčí, including several villages, from the Order of the Teutonic Knights and gave it to the Brotherhood.
In 1252 Agnes founded a new hospital with the Church of the Holy Spirit (later St. Francis) on the Old Town side of the Judith Bridge ("ad pedem pontis pragensis").
On 29 November 1378, the day of Charles IV's death, a fire broke out in the Order hospital of St. Francis, destroying the church and the convent with all its valuables, documents and privileges.
After the Hussite Wars, the Knights of the Cross with the Red Star actively participated in the recatholization of the country, and during the Jagiellonian period it flourished again and managed to regain some of its property.
From 1460 to 1490, Nikolaus Puchner became its twentieth Grand Master and commissioned a new main altar with a statue of the Virgin Mary (Assumpta) and scenes dedicated to the history, mission and spirituality of the Order.
The paintings on the wings of the altar are influenced by Dutch naturalism (Dieric Bouts), evident in the careful drawing of small details, the portrait-like characterization of faces, and the realism of the time, which brings the depicted action into the viewer's present and interprets it as an actual story.
[3] The characteristic features of the painting style are space depicted in the form of scenery flats, with an implausible perspective with multiple focal points, some naturalistic elements of vegetation and architecture, non-dramatic scenes, and cool colours rendered in large continuous areas.
On the upper part of the left movable wing of the altar, St Agnes is depicted as a princess handing a model of the church to the first Grand Master of the Order, Albert of Sternberg.
The surviving left wing shows the patron saint of the country, St. Ludmila, together with St. Ursula, who was popular in the hospital fraternity as a protector against the plague; the missing right part may have depicted St. Wenceslas.