The Puelioideae is a subfamily of the true grass family Poaceae with two genera, Guaduella and Puelia, each in its own tribe.

[1] Its members grow in the understory of rainforests.

This subfamily is one of the earliest-diverging grass lineages, sister to the big BOP and PACMAD clades:[2] Anomochlooideae Pharoideae Puelioideae BOP clade PACMAD clade

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