The park covers an area of 1,878 square kilometres (725 sq mi), and protects a part of the longest undeveloped shoreline anywhere on the Great Lakes.

Many plants that grow inside Pukaskwa National Park are typically found in Arctic alpine regions,[5] including encrusted saxifrage, birds-eye primrose, and butterwort.

Pukaskwa is home to the northernmost populations of Pitcher's thistle in Canada, with four colonies of the plant existing and self-sustaining on the coastal dune areas within the park.

Eastern white cedar, Labrador-tea, and Chaga all grow within Pukaskwa's area and can be boiled into tea for physical and spiritual health.

[8] Black bears, moose, beavers, peregrine falcons, river otters, lynx and timber wolves are found in this park.

[14] Thousand year old rock structures known as Pukaskwa Pits which were created by the original inhabitants of this area can be found on many of the park's cobblestone beaches.