It runs from Mount Pulai in Kulai District until Tanjung Pelepas, draining into the Tebrau Straits.

At its mouth lies the single largest seagrass bed in Malaysia,[2] which extends all the way to Pulau Merambong.

[3] The site is being studied to help manage the vast mangrove ecosystem, with assistance from University of Technology Malaysia and the National University of Malaysia, in line with the Integrated Management Plan for the sustainable use of mangroves in Johor.

[4] Sungai Pulai is also a Ramsar site, one of the few locations in the world recognized as a wetland of international importance.

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