Pusiano (Brianzöö: Püsian [pyˈzjãː]) is a comune (municipality) in the Province of Como, in the Italian region Lombardy, located about 40 kilometres (25 mi) north of Milan, and about 15 kilometres (9 mi) east of Como.

As of 31 December 2004, it had a population of 1,225, and an area of 3.2 square kilometres (1.2 sq mi).

In the 19th century, Pusiano became known for a small millennial cult founded by Angela Isacchi (1827-1895) and her sister Teresa (1833-1890).

The “prophetesses of Pusiano” proclaimed in the 1850s that the messages they were allegedly receiving from God (the “Holy Word”) were the third testament of the divine revelation, and that the Papal States and the Austrian Empire would both disappear if they would not officially recognize the new revelation.

The Isacchi sisters were supported by some local priests in Pusiano, while cardinal Andrea Carlo Ferrari in Milan and the Vatican regarded them as just mentally disturbed.