[3] The program is a comeback battle between 6 trending girl group acts, in order to "determine the real number one" when all 6 release their singles at the same time.

[5] The first and second seasons lasted for 10 weeks, both featuring 3 preliminary performances and 1 live comeback stage from each act.

The Comeback Singles, which are newly produced songs of the six acts, are released a few days prior to the final episode.

The digital points accumulated for the Comeback Singles and live votes for the comeback stages on the final episode, together with the accumulated points from the 3 preliminary rounds, were combined to determine the final winner.

[7] The comeback show for the second season's winner WJSN took place on July 5, 2022 with their first special single album Sequence.

Hosts Contestants Hosts Contestants[10] For the first season, the overall rankings are calculated out of a maximum of 100,000 points for each act, and distributed based on: For the preliminary performances, if one act gets a 6th place twice in a row, they will be eliminated from the show.

Before the performance, the six teams have ten minutes to arrange their cue sheet, which they must complete within the timing, otherwise, they will be deducted 1,000 points.

The team that receives the most votes for the Special Judge Award will have all 2,000 points secured to themselves (Monopoly Rule).

Each act must also perform a Queendom Quest, which are picked randomly, act that successfully completed the Queendom Quest, get one of the following benefits that are picked randomly: For the second preliminary round, the same points breakdown from the first round shall apply, wherein a maximum of 10,000 points can be earned, and the points are distributed based on: For the Self-Assessment, each contestant has to vote for a team that they believe to have performed better than them (Up vote) and a team that they believe to have performed worse than them (Down vote).

The team that receives the most votes for the Special Judge Award will have all 2,000 points secured to themselves (Monopoly Rule).

For part one of the third preliminary performances, the points breakdown are calculated out of maximum of 5,000 points for each act, and distributed based on: The votes breakdown are calculated by the Audience Judge Squad who will each vote for their favorite Vocal Unit and favorite member of the Performance Unit.

Before the performance, the act that ranked first in the third preliminary round gets to arrange their cue sheet for the finale.

[43] It was confirmed to be aired beginning at the end of April, with the show being hosted again by Lee Da-hee and Jang Sung-kyu.

[44] On March 20, Mnet confirmed the show's lineup would consist of Pentagon, ONF, Golden Child, The Boyz, Verivery, Oneus and TOO.

[46] On January 26, 2023, Mnet announced a spin-off of the series titled Queendom Puzzle, scheduled to premiere in the first half of 2023.