[2] On March 20, 1990, sixty LGBTQ people gathered at the Lesbian, Gay, Bisexual and Transgender Community Services Center in New York's Greenwich Village to create a direct action organization.

The goal of the unnamed organization was the elimination of homophobia, and the increase of gay, lesbian and bisexual visibility through a variety of tactics.

[3] The direct-action group's inaugural action took place at Flutie's Bar, a straight hangout at the South Street Sea Port on April 13, 1990.

Through parodying straight behavior (such as "spin the bottle") at these events, queer people refused to be invisible while publicly questioning the naturalized status of heterosexual coupling activity.

was arranged by members of Queer Nation after the lead singer, Turbo Harris[16] was accused of assaulting Boston club owner, Dennis Moreau.

[18][19] In the fall of 1990 the group helped organize a protest against a visiting televangelist who vowed to "exorcise the demons" from San Francisco on Halloween.

Other more radical actions include a blockade of Ventura Blvd, confrontations with various church groups in the area, and the taking over of a political science class at Los Angeles City College.

[26] The organization demonstrated in front of the ROTC building at the University of Nebraska–Lincoln in April 1991 to protest the military's policy of excluding LGBT people.

[27] Later in 1991, Queer Nation Nebraska produced a TV show consisting of two men kissing in a bathtub and pouring milk onto each other.

Other actions by the group included a march in the suburban town that was the home of Broussard's killers, seizing the rotunda of Houston City Hall after another murder, protesting the Houston Post's firing of columnist Juan Palomo after he came out, and protesting discrimination against HIV-positive nurse Brian Bradley.

"[citation needed] Queer Nation Utah was founded in January 1991 by Curtis Jensen, Melanie Bailey, and Connell O'Donovan.

O'Donovan went to San Francisco in December 1990 to attend Queer Nation meetings there in order to learn about the organization and gather materials and ideas to share back in Utah.

Kiss-ins were held at two predominantly heterosexual venues (Denny's diner and a Gay-owned dance club) where homophobic incidents had occurred.

They were physically attacked by a large crowd outside of an Andrew Dice Clay comedy concert that Queer Nation Utah was protesting.

[29] The Queer Nation chapters in Atlanta, Georgia; Columbia, South Carolina; Berea and Lexington, Kentucky; and Nashville, Tennessee founded by Kelvin Lynn Cothren and Cheryl Lynn Summerville were active in protesting known homophobic policies of the Cracker Barrel a restaurant chain in 1992.