Río Jatibonico del Sur is a river of southern Cuba.

The river's source is located approximately 1 kilometre south of the town Iguará at 220 metres elevation.

The water then splits into two streams, running down from the heights of the municipality of Santa Clara .

The river's mouth is located at the tip of Jatibonico in a low and marshy area, about 15 kilometers south of the town El Jíbaro.

The river has a total length of 119.5 kilometres, constituting the border between the municipalities of Taguasco and Jatibonico, as well as the latter and La Sierpe.