Rugby arrived in Tuscany with university students in 1931, with a team called GUF Firenze being founded[1] and for most of the pre-war period played in the National Division, and after the war were promoted to the then top level of Italian rugby becoming CUS Firenze, continuing to play in the upper division until 1983-84.

On June 25, 2015 the foundation of the RC I Medicei was announced at a press conference held at the Padovani sports centre.

The debut tournament of the new club, in Serie A 2015-16, saw the Medici finish second in their group in the first phase and fourth in the following promotion pool.

It was in the following tournament that the team managed to improve, repeating second place in the first phase but winning its own pool promotion in front of a top-flight of the Top12 competition.

The I Medicei squad for the 2019–20 season is:[4] Props Hookers Locks Back row Scrum-halves Fly-halves Centres Wings Fullbacks