The main line is the portion of the Trans-Caspian railway that connects Tashkent with the Amu Darya.

There are rail links with Kazakhstan, Kyrgyzstan (see Trans-Caspian Railway), Tajikistan, Afghanistan, and Turkmenistan.

Also Tajik trains of Dushanbe-Moscow (No: 319), Moscow-Dushanbe (No: 320), Khujand-Saratov (No: 335), Khujand-Atyrau (No: 335), Saratov-Khujand (No: 336), Khujand-Moscow (No: 359), Moscow-Khujand (No: 360), Kanibadam-Bokhtar (No: 389), Bokhtar-Kanibadam (No: 389) and Atyrau-Khujand (No: 692) passes from Uzbekistan.

With only one change of trains in Moscow, passengers can travel overland from Central and Western Europe (Berlin, Cologne, Vienna, Prague, Budapest, Helsinki, etc.)

[4] In March 2018, Uzbekistan Railways began a new service, connecting Tashkent with Balykchy.