Regina Maršíková (born 11 December 1958) is a retired tennis player from Czechoslovakia, present-day Czech Republic.

She had career wins over Martina Navratilova, Billie Jean King, Evonne Goolagong, Hana Mandlíková, Tracy Austin, and Virginia Wade.

Her best performances in Grand Slam singles events included three consecutive semifinals at the French Open from 1977 to 1979.

Maršíková was involved in an automobile accident in September 1981 near Prague, Czechoslovakia that caused a fatality.

Maršíková rejoined the WTA Tour in April that year at the Tournament of Champions in Florida.