A sister company of the same name, RegioJet a.s., with its registered office in Bratislava was established in 2010 and is nowadays an operator of rail and bus transport in Slovakia.

SInce 1996, Student Agency operated international bus transport – the first line was from Prague to London, and in the following years buses operated to other European countries, including cities in Germany, Switzerland, Benelux, lines connecting the Czech Republic with cities in Sweden, Norway, Italy, Hungary, Austria and the Slovak Republic, and later in France and elsewhere.

[6] In the early stages, during the operator's time as Student Agency, bus transport was provided by vehicles of Spanish brand Ayats Atlantis on the MAN chassis.

The transport was meant to be cheaper and more luxurious than the Pendolino from České dráhy, with a design resembling the ICE 3 trains operating in Germany.

The first train started on 24 April 2010 within the advertising event Žluté jaro na železnici (“Yellow spring on the railway”).

This advertising operation was described by a representative of the company as the following: “We want to show how modern vehicles can contribute to the simplification and improvement of transport service”.

RegioJet declared this step as unfair competition and a misuse of the dominant position of České dráhy on the market.

[14] In December 2012, RegioJet announced a CZK 30 – 50 mil loss for 2012, which was justified according to the "predatory pricing" by České dráhy on the route between Prague-Ostrava.

Before commencing transportation on this route, RegioJet reportedly invested approximately €60,000 into the modernisation of waiting shelters and other furniture at stations, as well as adding security cameras.

At present (since 2 September 2018), RegioJet trains run 4 times a day to Bratislava, and the other two lines on this route ensure transportation with the terminal in Brno.

Since December 2017, the transporter has cooperated with Graz-Köflacher Bahn und Busbetrieb GmbH to operates four pairs of trains from Prague through Brno to Vienna.

In both Standard and Relax classes, passengers receive a daily newspaper and magazines, coffee and bottled water free of charge and at the same time may order other paid-for snack items from an onboard menu.

After the purchase, the locomotives were modified for operation in the Czech Republic; the gear ratio was changed in order to increase the maximum speed of 140 km/h.

In addition, there is signate to denote the class, respective fares and pictograms of the offered services close to the entrance door.

In June 2011, RegioJet purchased 14 used coaches from Austrian operator ÖBB, which had been previously reconstructed to reach speeds of 200 km/h and retrofitted with AC, vacuum toilets and power sockets.

[31][32] They were transported to the Czech Republic in early June 2011 and late July 2011, before entering service in September 2011 having undergone a complete interior adjustment.

In September 2014 at InnoTrans, the Slovak company Molpir, the supplier of the FUNTORO on-board entertainment system, introduced a high-capacity coach for RegioJet from Romanian manufacturer Astra Vagoane Călători.