[3] The species is native to a small portion of the southeastern coastal plain of the United States in the western panhandle of Florida and extreme southwestern Georgia.

The skin is smooth and the dorsal surface is reticulated, with thin grey lines forming a net-like pattern on a brownish-black background.

This salamander is similar in appearance to Ambystoma cingulatum but the latter has a more frosted dorsal pattern and larger white spots on the ventral surface.

[9] Ambystoma bishopi is a burrowing species of salamander and lives among the leaf litter beneath longleaf pine (Pinus palustris) and wiregrass (Aristida stricta) in the flatwoods coastal plain ecosystems of the Southeastern United States.

Eggs are laid in small depressions under herbaceous vegetation or leaf litter, at the base of stumps, in the entrances of crayfish holes, or in other hidden places near ponds.