[2] A former general practitioner, Di Natale became federal parliamentary leader of the Australian Greens on 6 May 2015 following the resignation of Christine Milne.

[10] In both 2002 and 2006, Di Natale was narrowly defeated in the seat of Melbourne in the Victorian Legislative Assembly, almost unseating ALP health minister Bronwyn Pike.

[13] In April 2007, Di Natale spoke out about the health implications of climate change,[14] and later that year voiced concerns about terror laws in relation to the then suspect Muhamed Haneef.

Di Natale was elected unopposed as parliamentary leader of the Greens party room on 6 May 2015 following the resignation of Christine Milne from the position.

[19][20] Di Natale has written in favour of legalising cannabis and against the war on drugs, as well as his support for a universal basic income.

[23] Di Natale also helped establish Senate inquiries into a number of issues of public significance including budget cuts, medicinal cannabis, the emergence of "superbugs",[24] hospital funding,[25] air pollution,[26] pharmaceutical transparency,[27] sports science[28] and gambling reform.

[29] Di Natale conducts ongoing campaigns for improved human rights in Indonesia's West Papua,[30] timely access to cost-effective drugs through Australia's Pharmaceutical Benefits Scheme,[31] and science-based public health policies in areas such as wind farms[32] and vaccination policy.

[37] Lidia Thorpe was selected by Greens members to fill the Senate vacancy caused by Di Natale's resignation.

[42] Di Natale, his wife and two sons live on a hobby farm in the foothills of Victoria's Otway Ranges.