He played college football for the University of Florida, where he was a member of two BCS National Championship teams.

He was the first Gator freshman wide receiver to have three touchdown catches in a game since Jabar Gaffney accomplished the feat in 2000. Cooper saw action in Florida's triumphs over Alabama, Tennessee and Kentucky, mostly on special teams.

Cooper hauled in a career-high and team-best four catches for 122 yards and two touchdowns, including a career-long 59-yard grab in his 2007 debut against Western Kentucky.

[5] Cooper appeared in fourteen games for the Gators in his junior season, starting all but two (against Hawaii and Florida State).

He hauled in a key touchdown, along with a season-high 51-yard reception, that put Florida in scoring position during the SEC Championship Game against Alabama.

He totaled 28 yards on two receptions in the BCS National Championship Game, including a crucial 17-yard catch from Tim Tebow on 3rd-and-12.

He was scheduled to get a physical and receive half of his $250,000 signing bonus on January 17, but declined and told the Rangers that his focus was football, thus voiding his baseball contract.

[12] He scored his second touchdown of his career against the New York Giants in week 10 of the 2011 NFL season on an 8-yard completion from Vince Young.

Cooper had a career game in Week 9 against the Oakland Raiders, where he posted 5 receptions for 139 yards (27.8 avg) and 3 touchdowns.

After a slow start, Cooper put together a solid season with 47 receptions for 835 yards and 8 touchdowns, all career highs.

In July 2013, a video surfaced of Cooper being denied backstage access at a Kenny Chesney concert in the previous month.

"[22] In the aftermath, the Philadelphia Eagles fined Cooper an undisclosed amount, but still allowed him to resume activities with the team.