The main entrance is located about 30 miles (48 km) north of Orlando in Sorrento, 3 miles (4.8 km) west of the Wekiva River bridge on State Road 46 and extends into Orange and Seminole Counties to the south.

Activities include bicycling, hiking, canoeing, kayaking, horseback riding, hunting and wildlife viewing.

Amenities include a canoe and kayak launch, rentals, swimming, tubing, about 17 miles (27 km) of trails, access to the Rock Springs Run and the Wekiva River, primitive canoe and equestrian camping facilities.

The 9 mile Rock Springs Run starts at Kings Landing in Apopka, runs through Rock Springs Run State Preserve, and meets the Wekiva River about a half mile downstream from Wekiwa Springs State Park.

The hyacinth siltsnail (Floridobia floridana) is found in northern Florida and on Cumberland Island, Georgia.