The Roman Catholic Diocese of Comayagua is a Latin suffragan bishopric in the ecclesiastical province of the Archdiocese of Tegucigalpa.

The present diocese, erected 13 March 1963, revives a larger colonial bishopric.

Like many missionary dioceses, it had mainly regular priests as Ordinaries, who generally died in office or were transferred/promoted within the vast Spanish colonial empire.

On 1916.02.02 it was suppressed, its territory being divided to establish the Archdiocese of Tegucigalpa (its Metropolitan still, in the national capital), the then Apostolic Vicariate of San Pedro Sula and the Diocese of Santa Rosa de Copán.

It was restored on 1963.03.13, as Diocese of Comayagua, on much smaller territory (7,527 km2) split off from its Metropolitan, the Archdiocese of Tegucigalpa.