The Roman Catholic Diocese of Daule (Latin: Dioecesis Dualensis) is a Latin Church ecclesiastical jurisdiction or diocese of the Catholic Church located in the city of Daule, Ecuador.

The diocese covers an area of the 12 cantons in the Guayas Province: Daule, Balzar, Colimes, El Empalme, Isidro Ayora, Lomas de Sargentillo, Nobol, Palestina, Pedro Carbo, Samborondón, Santa Lucía and Salitre.

[citation needed] On 2 February 2022, Pope Francis established the Diocese of Daule, when it was split off from the Archdiocese of Guayaquil.

[1] As its first ordinary he named Giovanni Battista Piccioli, auxiliary bishop of Guayaquil.

[3] On 17 March 2022, Pope Francis accepted Piccioli's resignation and named Luis Cabrera Herrera, Archbishop of Guayaquil, apostolic administrator.