The Diocese of Huelva (Latin: Dioecesis Onubensis) is a Latin Church diocese of the Catholic Church located in south-western Spain, and its borders coincide with those of the civil province of Huelva, part of the autonomous community of Andalusia.

The territory includes four different zones: The economy is based on agriculture, fishing and mining.

In the Diocese are located several historic places where Christopher Columbus sailed out of on his first voyage in 1492.

The see of the diocese, and provincial capital is the city of Huelva (pop 150,000), located next to the estuary that is created by the confluence of the rivers Tinto and Odiel, opening itself to the Atlantic near the city.

The Diocese was erected 22 October 1953, dismembered from the Archdiocese of Seville.