The Diocese of Valparaíso (Latin: Vallis Paradisi) is a suffragan Latin diocese in the ecclesiastical province of Santiago de Chile in central Chile.

Its cathedral episcopal see, the Catedral de Santiago, dedicated to the Apostle St. James the Elder (Spanish: Santiago) in the city of Valparaíso, Valparaíso Province, is a Minor World Heritage Site.

As per 2014, it pastorally served 966,000 Catholics (74.1% of 1,304,000 total) on 4,763 km² in 69 parishes and 260 missions with 171 priests (86 diocesan, 85 religious), 71 deacons, 406 lay religious (133 brothers, 273 sisters) and 16 seminarians.

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