The Roman Catholic Diocese of Villarrica del Espíritu Santo (Latin: Dioecesis Villaricensis Spiritus Sancti) is a Latin Rite suffragan diocese in the ecclesiastical province of the Metropolitan Archdiocese of Asunción, which cover all and only Paraguay.

Its cathedral episcopal see is Catedral Santa Clara, dedicated to Saint Clara, in the city of Villarrica, Guairá Department.

As per 2014, it pastorally served 342,000 Catholics (99.7% of 343,000 total) on 13,342 km² in 35 parishes, 43 priests (39 diocesan, 4 religious), 65 deacons, 89 lay religious (13 brothers, 76 sisters), 27 seminarians.

(all Roman Rite and natives)