[2][citation needed] Three types of rosette techniques have been developed and used experimentally.
[citation needed] The Rosette test is performed on postpartum maternal blood to estimate the volume of fetal-maternal hemorrhage in case of an Rh negative mother and an Rh positive child.
[4] Upon addition of enzyme-treated cDE indicator cells, the presence of Rh positive fetal blood causes rosetting, which can be seen by light microscopy.
[4] The test is recommended for Rh negative mothers within 72 hours of giving birth to an Rh-positive infant.
[citation needed] Erythrocyte antibody complement rosetting (EAC-rosetting), occurs when antibody in the presence of complement is bound to the surface of a red blood cell.