Rostov Nature Reserve (Russian: Ростовский заповедник) (also Rostovsky) is a Russian 'zapovednik' (strict nature reserve) that protects a variety of sensitive southern European steppe wetlands, the largest herd of wild horses in Europe (the Don Mustangs), and also wetland habitat for birds.

[1][2] The Rostov Nature Reserve is located in the south of the East European plain, in the Manych River valley.

The dominant geological feature of the reserve is Lake Manych-Gudilo, a saltwater reservoir which is surrounded be dry steppe and semi-desert landscape.

This climate is characterized by large swings in temperature, both diurnally and seasonally, with warm summers and cold winters, and at least one month averaging over 22 °C (72 °F).

Residents include the bustard, little tern, Pygmy Cormorant, pink and Dalmatian pelican, spoonbill, black-headed gull, owl, and flamingos.

The other trail, "Azure Flower", tours fields of tulips and iris and features large flocks of cranes and gulls.