Rouseville is a borough in Venango County, Pennsylvania, United States.

As of the census[5] of 2000, there were 472 people, 204 households, and 137 families residing in the borough.

There were 240 housing units at an average density of 264.6 per square mile (102.2/km2).

There were 204 households, out of which 22.1% had children under the age of 18 living with them, 48.5% were married couples living together, 13.2% had a female householder with no husband present, and 32.4% were non-families.

29.9% of all households were made up of individuals, and 18.1% had someone living alone who was 65 years of age or older.