Route 666 is the second album by the all-female tribute band The Iron Maidens and the first under their Japanese label Powerslave Records.

Produced by Bob Kulick and Brett Chassen (who worked on the 2005 Iron Maiden tribute album Numbers from the Beast), Route 666 is a CD/DVD package, with the DVD featuring the band's live performance at the Galaxy Theatre in Santa Ana, CA, on March 12, 2005.

Most notable are the guest appearances of Motörhead guitarist Phil Campbell and Iron Maiden keyboardist Michael Kenney.

Liner notes in the sleeve include commentary by Bob Kulick and Show-Ya vocalist Keiko Terada.

To promote the CD in Japan, the band filmed a music video for "Fear of the Dark", which is featured on their official Japanese website.