[1][5] Nicolai presented the quiz show Rat van Fortuin for broadcasting association BNN in 2006 and 2007.

[12][13] For the show, Nicolai took part in various activities and challenges, including playing a role in a 2006 episode of the American soap opera As the World Turns.

[18] In the same year, Nicolai and Karoline Kamosi presented the fourth season of the show 71° Noord in which contestants travel through Norway.

[26][27] He presented the 2013 television show Golden Oldies in which an elderly choir sings rock songs.

[28] Nicolai presented De allerslechtste echtgenoot van Nederland, a 2013 show in which husbands compete against each other.

[32] Nicolai and Sophie Hilbrand presented the 2015 edition of De Nationale IQ Test.

[33][34] At the end of March 2015, Nicolai left BNNVARA, the broadcasting association which formed in January 2014 as a merger of BNN and VARA.

[40][46] In 2016, Nicolai and Wendy van Dijk presented the quiz show De Nationale Gezondheidstest with questions about health.

[40][54] In the show, science journalist and presenter Diederik Jekel helps to explain the scientific concepts and experiments.

[57][58] In the same year, Nicolai and Tijl Beckand became team captains in the show Wie ben ik?.

[61][62] Since 2019, Nicolai presents the quiz show Ik weet er alles van!

[74][75] Gerard Joling and Ruben Nicolai presented the television show Te land, ter zee en in de lucht.

[78][79] He also presents the television show De Erfgenaam in which he searches for people that can receive an unclaimed inheritance.