The São Bartolomeu River Basin Environmental Protection Area (Portuguese: Área de Proteção Ambiental da Bacia do Rio São Bartolomeu) is a protected area in the Federal District, Brazil.

It was created on 7 November 1983 and is administered by the Chico Mendes Institute for Biodiversity Conservation.

[1] The environmental protected area has territory distributed over the administrative regions of São Sebastião, Jardim Botânico, Paranoá, Itapoã, Sobradinho and Planaltina, in the Federal District.

The purpose is to protect biological diversity, control human occupation and ensure sustainable use of natural resources.

[2] The area is an important corridor between the Águas Emendadas Ecological Station and the protected environment areas of Cafuringa, Lago Paranoá and the Gama and Cabeça-de-Veado basins.