The line is operated at 20-minute intervals between Dachau and Markt Schwaben.

It is operated using class 423 four-car electrical multiple units, usually as two coupled sets.

In the evenings and on Sundays they generally run as single sets.

Extra peak hour services are operated between Dachau and Altomünster, using class 420 four-car electrical multiple units (this is the only place this class operates on the Munich S-Bahn network), creating a 30-minute frequency on the branch.

The service runs over lines built at various times: The S2 was introduced on 28 May 1972 and ran between Petershausen and Deisenhofen.