In the game, the player controls a human test subject, D-9341, who is trapped in an underground facility designed to study and contain anomalous entities known as SCPs.

The player controls D-9341, a test subject who must navigate the facility while avoiding various SCPs and Mobile Task Force (MTF) units.

In addition to navigating the facility and avoiding SCPs, the player can find various items to help with survival, such as gas masks, keycards, and other tools.

The plot of the game follows the player as they attempt to escape the SCP Foundation's containment facility after a breach occurs, releasing several dangerous SCPs.

[2] At the start of the game, D-9341 is one of three test subjects involved in the study of SCP-173, a statue-like entity that can move incredibly quickly when not in the direct line of sight of a human.

As the player explores, they encounter other dangerous SCPs, including SCP-106 (a decaying humanoid that can drag victims into a pocket dimension), SCP-096 (a creature that will kill anyone who views its face), and SCP-079 (a sentient AI responsible for the facility's power failure).

[4] Rikkonen focused heavily on creating an immersive atmosphere, emphasizing environmental design and sound to enhance the horror experience.

Rock, Paper, Shotgun compared the game to the TV show Warehouse 13 but noted that SCP – Containment Breach offered a much more intense experience, focusing on fear rather than humor.