The southern tournament is open to full member non-league clubs in the south of the country, while similar stipulations applied to its northern counterpart.
To compensate clubs for the demise of the tournaments, two new ones were put in their place.
The reserve teams of Stirling University and Stranraer, as well as Celtic B and Hearts B, do not take part.
It is currently an all-in draw from the start, having previously seen the opening rounds regionalised at times during the competition's history.
The competition is named the CarMatKings South Challenge Cup for sponsorship reasons in 2024–25.