As female and male astronauts became more prominently integrated with the shuttle program, NASA enacted an unwritten rule that husband/wife couples would not be assigned to the same mission.

[1] When asked at a NASA press conference if intimate activities would be taking place on the mission, Davis denied that possibility.

[2] At the beginning of September, a problem with an oxygen line in the shuttle's main propulsion system was identified, however, it was resolved without forcing a postponement of the mission.

[11][12] Aboard the Spacelab Japan module, Jemison tested NASA's Fluid Therapy System, a set of procedures and equipment to produce water for injection, developed by Sterimatics Corporation.

She then used IV bags and a mixing method, developed by Baxter Healthcare, to use the water from the previous step to produce saline solution in space.

[16]: 4  External experiments using the Ultraviolet Plume Imager (UVPI) on the LACE satellite and observations at the Air Force Maui Optical Station (AMOS) were also conducted while Endeavour was in orbit.

Among the GAS canisters was G-102, sponsored by the Boy Scouts of America's Exploring Division in cooperation with the TRW Systems Integration Group, Fairfax, Virginia.

A second experiment to investigate how Liesegang rings formed in space failed to operate correctly due to friction in parts of the mechanism.