Saint Anne with the Virgin is a tempera painting attributed to the Greek painter Angelos Akotantos.

According to the Institute of Neohellenic Research, fifty paintings are attributed to Angelos Akotantos.

The Italian painters adopted oil painting opposing the egg tempera technique.

Giorgio Vasari's famous book Lives of the Most Excellent Painters, Sculptors, and Architects commented on the technique.

[6] The bright coloration reflects the painter's knowledge of the Italian Renaissance cangiante technique.

The bright garment Anne is wearing features ornate gold patterns.

The young Virgin Mary looks up at her mother, and in her right hand she holds up a sacred lily.

Gabriel told the Virgin that she had "found favor with God" and would bear a son and name him Jesus.