According to his Hagiography, Colluthus' father was the governor of the Upper Egyptian city of Ansena.
He lived a celibate, although his sister was married to Arianus, who became Ansena's governor after the departure of Colluthus' father.
When Roman emperor Diocletian began his persecutions of Christians, Arianus apostatized in order to keep his position as a governor.
After three years, a different governor of Oxyrhynchus took Colluthus out of prison, tortured him, and eventually cut his head off on 25 Pashons.
After Christianity was declared one of the Empire's legal religions by the emperor Constantine I, a church was built for Saint Colluthus, where his relics were placed.