Sainte-Colombe (French pronunciation: [sɛ̃t kɔlɔ̃b] ⓘ) is a commune in the Doubs department in the Bourgogne-Franche-Comté region in eastern France.

The commune lies 7 km (4.3 mi) northeast of Pontarlier in the valley of the Drugeon.

The commune has a railway station, Sainte-Colombe, on the Frasne–Les Verrières line.

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