Sainte-Vertu (French pronunciation: [sɛ̃t vɛʁty]) is a commune in the Yonne department in Bourgogne-Franche-Comté in north-central France.

The surrounding communes are Môlay, Poilly-sur-Serein and Aigremont.

Sainte-Vertu is situated 27 km (17 mi) to the south-east of Auxerre which is the nearest large town.

The Morvan natural park is approximately 29 km (18 mi) away.

Recorded population peaked at 544 in 1821, but has fallen back steadily subsequently, the decline accelerating with the mechanisation of agriculture in the twentieth century.