From the start the aim of the choir was to perform choral music written for boys' and men's voices from the Middle Ages to the 20th century.
On Carl Nielsen's recommendation the City Council of Copenhagen in 1929 established a Choir School for the boys, the present Sankt Annæ Gymnasium.
Its repertoire ranges from commissioned works by contemporary composers , such as Poul Ruders, Bernhard Lewkovitch, Per Nørgaard, Vagn Holmboe and Palle Mikkelborg, back to the earliest polyphony.
The choir has also taken part in productions for radio and television both in Denmark and in other countries, with CDs being made on labels such as EMI, Decca, Vanguard, Chandos, Kontrapunkt and Danica Records (OH).
In 2003, the Copenhagen Royal Chapel Choir was awarded the Liliane Bettencourt Choral Singing Prize in partnership with the Académie des beaux-arts.