Sant'Eustachio ([santeuˈstaːkjo]) is a Roman Catholic titular church and minor basilica in Rome, named for the martyr Saint Eustace.

The church was recorded as a diaconia (a centre for helping the poor and the sick) at the end of the pontificate of Pope Gregory II (715-731).

The new high altar, in bronze and polychrome marble, was added by Nicola Salvi in 1739 and in 1749 Ferdinando Fuga put a baldachin over it.

On top of the pediment stands a deer head with a cross between the antlers (done by the sculptor Paolo Morelli († 1719), in reference to the legend of Saint Eustace.

The top of the altar rests on an urn in porphyry rosso antico, the costly stone of the ancients, that contains the putative relics of Saint Eustace.

It represents the martyrdom of Saint Eustace and his family who were roasted to death inside a bronze statue of a bull or an ox, in the year AD 118.

The gilded balustrade and the wooden front of the organ were executed in Rococo style by Bernardino Mammucari, Francesco Michetti and Carlo Pacilli.

Above the organ stands a glass window representing "the Penitent Magdalene", realized in the last decade of the 19th century by Gabriel and Louis Gesta di Tolosa.

The pulpit was executed in polychrome marble and dates from 1937 to commemorate Pope Benedict XV (1914-1922) who as Cardinal Giacoma della Chiesa frequently preached in this church.