Santi Giovanni e Petronio dei Bolognesi is a Roman Catholic church in central Rome, Italy.

It is named after the Saints John the Evangelist and Petronius, who are patrons of the city of Bologna.

It is located in the Rione of Regola, on Via del Mascherone, across the street and just south of the Gardens behind the Palazzo Farnese.

Most of the artists originally decorating the church were Bolognese, but many of the paintings were removed and some are lost.

The main altarpiece with a Virgin, Baby Jesus, and Saints John the Evangelist and Petronio was painted by Domenichino; it is now in the Galleria Nazionale d'Arte Antica in Palazzo Barberini of Rome.