In the east, it extends down the Sassafras Gully, whilst to the West much of the locality is within the Dandenong Ranges National Park.

Both creeks provide important corridors for native flora and fauna, and are both largely protect by parklands.

Woori-Yallock Creek then flows northward to eventually join the Yarra River next to the Warramate Nature Conservation Reserve.

Sassafras Creek's Riparian Zone is almost entirely protected by public land, which in this case is Dandenong Ranges National Park.

The Dandenong Ranges Tourist Track, a walking trail, runs for 15 km alongside the Sassafras Creek, past Kallista and Monbulk to Emerald.