Schaeffer–Fulton stain

[1] The primary stain is malachite green, and the counterstain is safranin, which dyes any other bacterial bodies red.

After cooling, the slide is decolorized with water and counterstained with safranin.

Using an aseptic technique, bacteria are placed on a slide and heat fixed.

After cooling, the slide is rinsed with water for thirty seconds.

The procedure was designed by Alice B. Schaeffer and MacDonald Fulton, two microbiologists at Middlebury College, during the 1930s.