Opened on 28 March 1992, Scienceworks is housed in a purpose-built building "styled along industrial lines" near the historic Spotswood Pumping Station,[2] constructed in 1897, whose steam engines form an associated exhibit.

[3] Displays and activities offered by the museum include hands-on experiments, demonstrations, and tours.

Until late 2013, the 1883 clock tower from Flinders Street station was also located at the museum.

[6] The clock restored with an electric movement is now located at the Southern Cross station.

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