The film was written and directed by Don Mancini in his directorial debut, and stars Jennifer Tilly, Redman, Hannah Spearritt, John Waters, Billy Boyd and Brad Dourif.

Six years after the previous film, the benevolent and genderfluid living-doll child of Chucky and Tiffany is being held captive by "Psychs", an abusive British ventriloquist who exploits him for his act.

Shitface escapes Psychs and tracks the Chucky and Tiffany dolls to a prop room in Hollywood.

Tiffany knocks them out and uses a turkey baster to inseminate Jennifer with Chucky's semen, intending to use her baby as a host for Glen/Glenda's soul.

The next day, Jennifer wakes up to find herself with a full pregnant belly, a consequence of the voodoo magic.

Jennifer gives birth to twins, a boy and a girl, and Tiffany realizes that both Glen and Glenda can inhabit the two children.

However, the chaos causes Chucky to have an epiphany: he has finally accepted his life as a living doll and no longer wishes to become human.

Enraged, Chucky throws a knife at Jennifer to stop Tiffany from leaving him, but Stan jumps in the way, sacrificing himself to save her.

Meanwhile, Glen opens a birthday present to find Chucky's severed arm, which springs up and grabs him.

[9] Don Mancini, who is gay and was interested in exploring LGBT-related themes in the next film, decided to write a screenplay inspired by the 1953 cult classic Glen or Glenda in which Chucky's son is an innocent person suffering from gender dysphoria.

He also decided to continue the shift in the series towards comedy after noting that horror villains such as Michael Myers, Jason Voorhees, and Freddy Krueger became less scary as they became more familiar with audiences.

[4] In Australia, Seed of Chucky opened at #8 with $260,958 for the week of February 6–8, 2005 behind Million Dollar Baby (#2), and Sky Captain and the World of Tomorrow (#6).

On Rotten Tomatoes, the film holds a 37% approval rating based on 78 reviews, with a weighted average of 4.60/10.

The critical consensus reads: "Give Seed of Chucky credit for embracing the increasing absurdity of the franchise — even if the end results really aren't all that funny or entertaining.

[18] Roger Ebert gave the film two stars out of four stating, "Seed of Chucky is actually two movies, one wretched, the other funny.