[3] The Seminole Gulf Railway has a commonly owned affiliated company, the Bay Colony Railroad Corp. (reporting mark BCLR), which is based in southeastern Massachusetts.

Freight transported by Seminole Gulf Railway includes lumber, propane, stone, steel, scrap metal, and other commodities.

In addition to carrying freight, Seminole Gulf Railway also operates a popular Murder Mystery Dinner Train from Fort Myers.

Under normal operation, the dinner train runs five nights a week, year-round from a station at Colonial Boulevard north to a point just south of Punta Gorda before returning.

The dinner train is often headed by a vintage EMD F-unit locomotive, SGLR 502, which previously operated on the Baltimore and Ohio Railroad and the Long Island Rail Road.

[6] Special holiday dinner trains also operate including Sweetheart Express on Valentine's Day, Halloween Ghost Train, New Year's Eve Gala, and Christmas Rail-Boat (which runs to the historic Punta Gorda Atlantic Coast Line Depot for a Christmas boat tour through Punta Gorda Isles) to name a few.

The first excursion train ran on January 5, 1991, which initially operated from a small platform in North Naples near Railhead Park.

The Fort Myers Division begins just north of Arcadia, where it continues south from CSX's Brewster Subdivision (at milepost SVC 880.75).

[11] From this point, it runs south across the Peace River, passing Morgan Park, and turns southwest into the Arcadia yard.

In Punta Gorda, it turns south and then southeast closely paralleling Interstate 75 towards North Fort Myers before crossing the Caloosahatchee River between there and Tice.

The northernmost section of the Seminole Gulf track along with the Arcadia yard and the CSX line to the north was originally built by the Charlotte Harbor and Northern Railway.

Built from 1907 to 1910, the Charlotte Harbor and Northern Railway (which would later be acquired by the Seaboard Air Line Railroad in 1926) historically extended from Arcadia south to Boca Grande.

The line from the Arcadia yard southeast along Pine Street was the Charlotte Harbor and Northern's connecting track to the Florida Southern Railway.

[17] In Punta Gorda, the line initially continued west through the city and terminated at a dock facility in the Peace River near Charlotte Harbor.

Charlotte Harbor was Henry Plant's ultimate goal and he had no interest in having the line continue south to Fort Myers.

The alignment through Tice was selected so the line would cross the wide Caloosahatchee River farther upstream at Beautiful Island, where a series of shorter trestles could be built.

[1] The northernmost trestle originally had a steel swing span built by the American Bridge Company to accommodate river traffic.

The spur that runs from the line just south of State Road 82 is all that remains of the wye and tracks that once served the downtown Fort Myers depot and docks.

[5] The Atlantic Coast Line relocated its freight yard from downtown Fort Myers to its current location south of Hanson Street in 1960.

The Baker Spur would extend beyond the route of Interstate 75 to serve rock mines in eastern Lee County on land owned by the Atlantic Land and Improvement Company (known today as Alico, Inc.), which at one point had been a subsidiary of the Atlantic Coast Line Railroad and was the holding company for its real estate division.

In the mid-2000s, a short stretch of the line between Edison Avenue and State Road 82 in Fort Myers was realigned and upgraded with continuous welded rail.

Track to Venice previously ran from the wye at the southeast corner of the current line and went south-southeast through Fruitville, Bee Ridge, Palmer Ranch and Nokomis.

The Atlantic Coast Line (ACL) came to the area later in 1924 as part of the Florida land boom when they built the Tampa Southern Railroad (the east track).

[31] As previously mentioned, the line was abandoned from Venice to Palmer Ranch in 2004 due to decreased demand and the heavily deteriorated condition of the tracks and bridges.