Separation Creek is a small coastal locality in the Shire of Colac Otway, Victoria, Australia.

[1] The village is situated at the mouth of the Separation Creek, on the Great Ocean Road, which follows the coast through the locality.

[4][5] The Wye River Separation Creek Progress Association is a local community organisation which aims to "advance the welfare and protect the environment" of the two towns.

Surf Life Saving Australia describes the beach as "suitable for a picnic or fishing, but unsuitable for safe bathing".

[9] On Christmas Day 2015 a bushfire destroyed at least 18 homes in Separation Creek.