Separation Sunday is the second studio album by the American indie rock band the Hold Steady, released on May 3, 2005, through Frenchkiss Records.

A concept album, Separation Sunday follows the interconnected stories of several fictional characters: Craig (the narrator), Holly (short for Halleluiah), a sometimes addict, sometimes prostitute, sometimes born again Christian or Catholic (and sometimes all three simultaneously); Charlemagne, a pimp; and Gideon, a skinhead, as they travel from city to city and party to party.

[1] Separation Sunday is lyrically dense, full of Biblical allusions,[2] self-reference[3] word play, and puns.

In a review of the album, Blender described the Hold Steady as "sound[ing] like the best bar band in the world.

"[5] The album cover was photographed at the corner of Maspeth Avenue and Conselyea Street in Williamsburg, Brooklyn.