Tsuboi began his single-seater racing career in 2012 and drove for two years in Formula Challenge Japan.

In 2015, he made his debut in the inaugural FIA F4 Japanese Championship with the TOM'S Spirit team.

[1] Tsuboi made three starts in the Macau Grand Prix FIA Formula 3 World Cup between 2016 and 2018.

He finished third in the 2017 GT300 standings, winning the Fuji 500 km and the overseas race at Chang International Circuit.

Tsuboi finished second in the opening round at Okayama International Circuit, battling Kenta Yamashita for the lead for several laps.

Heading into the final round at Fuji Speedway, Tsuboi and Sekiguchi trailed championship leader Naoki Yamamoto by 16 points.

Yamamoto was taken out from a championship-clinching position after GT300 driver Ren Sato crashed into his car with 15 laps remaining, which ultimately secured the championship for Tsuboi and Sekiguchi.

The pair won the Golden Week Fuji 450 km race, finished second in the spring race at Suzuka, then reeled off back-to-back wins at Autopolis and Mobility Resort Motegi to clinch the championship by a whopping 29-point final margin.

After his record-setting 2018 Japanese F3 campaign, Tsuboi made the step up to the Super Formula Championship in 2019, joining JMS P.mu/Cerumo-INGING.

[8] After five seasons at Cerumo-INGING, Tsuboi moved to Vantelin Team TOM'S in 2024 to replace his former Super GT co-driver Miyata.

[6] Tsuboi won his first driver title championship, with 3 race wins and 7 podiums to his name as he beats both Tadasuke Makino and former champion Tomoki Nojiri.