Mackinnon began her acting career in 1988, appearing in the Australian feature film Something About Love.
[3][5][6][7] Mackinnon's other television work includes roles on Water Rats, All Saints, Sir Arthur Conan Doyle's The Lost World, The Cut, Rescue: Special Ops, Cops L.A.C.
Mackinnon also played roles in three direct-to-video films, Python, Dark Waters and Submission, for which she also served as producer.
In June 2009, Mackinnon appeared as a presenter alongside host Karl Stefanovic and Scott Cam in Random Acts of Kindness on the Nine Network.
[8] On 30 April 2024, it was announced that Mackinnon will join the reality television series The Summit, as a contestant for its second season, which premiered on 12 May 2024 on Nine Network.
"[13] As a child traveling with her parents, she found an abandoned Australian Cattle Dog, and since then has had ACDs in her life.