The six-lined racerunner is found throughout much of the southeastern and south-central portion of the United States, from Maryland to Florida in the east, across the Great Plains to southern Texas and northern Mexico.

[3][4] In a study conducted on A. sexlineata in Mexico, the majority of the individuals found were inhabiting areas near the seashore that were formed and influenced from maritime climate and hurricanes.

[6] The six-lined racerunner is typically dark green, brown, or black in color, with six yellow or green-yellow stripes that extend down the body from head to tail.

[11] Due to its extensive range, A. sexlineata is found in a wide variety of habitats including grasslands, woodlands, open floodplains, or rocky outcroppings.

This act entails the male rubbing its cloaca and pelvic region rapidly on the ground, sometimes in a figure eight, while simultaneously moving forward.

[16][9] When he finds a receptive female, the male will straddle her, curve his body over hers, and bite her on the opposite side to where his cloacal region is in order to start copulation.

[18] There are three recognized subspecies of A. sexlineatus: The six-lined racerunner is listed as a species of concern in the states of Michigan and Wisconsin, due to its limited population but otherwise holds no official conservation status.

[20] Playa Bagdad in Mexico has seen a large decrease in six-lined racerunner populations due to recreational vehicle damage to sand dunes.