The glands are surrounded by tissue that swells with blood during sexual arousal, and secrete a fluid, carried by the Skene's ducts to openings near the urethral meatus, particularly during orgasm.

The Skene's glands' openings are located in the vestibule of the vulva, around the lower end of the urethra.

[2][3] Although there remains debate about the function of the Skene's glands, one purpose is to secrete a fluid that helps lubricate the urethral opening.

[3][4] In addition to PSA and acid phosphatase, Skene's gland fluid contains high concentrations of glucose and fructose.

[9][10] Female ejaculation and squirting (secretion of large amounts of fluid) are believed by researchers to be two different processes.