The team initially played in the Southern Alliance alongside the likes of Tottenham Hotspur and Windsor & Eton before later moving on to the Great Western Suburban League.

Slough transferred over to the Southern Football League Division One South & West for the 2007–08 season, where they finished 21st out of 22 teams.

[1] In the season of 2004–05, Slough Town beat Walsall to reach the 2nd round of the FA Cup however they then lost to Yeading.

Slough Town were also the Isthmian League Cup winners in 2004–05 after they beat Hampton & Richmond Borough 3–1 in the final with 2 goals from Ian Hodges and 1 from Josias Carbon to help them secure the win and the silverware.

Having beaten second-placed Hitchin Town 2–1 in the play-off semi-final, Slough lost 4–0 to Chesham United in the final, meaning they had to remain in the same division for the 2010–11 season.

The club looked to take a new direction in 2013–14 and appointed Neil Baker and Jon Underwood, the management duo who had taken league rivals Godalming Town to the play-offs in the previous season.

By virtue of an 89th-minute winner from Manny Williams, they won 2–1 and thus secured promotion to the National League South for the forthcoming season.

Slough Town started the 2016–17 season playing their early home games at Holloways Park, Windsor Road, Beaconsfield, Buckinghamshire.

They moved back into the borough of Slough for the first time in over a decade on 29 August 2016 when they played their first game at the new ground, Arbour Park, against Hayes and Yeading United, winning 2–1.

The proposed location for the development was the Arbour Vale school site on Stoke Road, to the north of the town.

[11] In 2018, after opening two years prior, Arbour Park hosted a number of games in the 2018 CONIFA World Football Cup.

Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.